Isolation and Identification of Lactobacillus delbrueckii from Cow and Sheep and its Effect on Pseudomonas putida Biofilm Formation: Isolation and Identification of Lactobacillus delbrueckii from Cow and Sheep

Haya Abdulkareem Albakry; Sawsan Mohammed Sorchee

doi:10.63841/iue32687

Authors

Haya Abdulkareem Albakry Department of Biology, College of Education, University of Salahaddin, Erbil, IRAQ Author
Sawsan Mohammed Sorchee Department of Biology, College of Education, University of Salahaddin, Erbil, IRAQ Author https://orcid.org/0000-0002-6676-1318

DOI:

https://doi.org/10.63841/iue32687

Keywords:

Lactic acid bacteria, Pseudomonas putida, Biofilm inhibition, Antibiotic susceptibility

Abstract

Lactic acid bacteria (LAB), especially Lactobacillus delbrueckii subsp. lactis and subsp. Bulgaricus are well-known for their probiotic and antimicrobial properties. Lactic acid bacteria (LAB) were isolated from yogurt samples and comprehensively characterized using phenotypic and molecular approaches, confirming their classification as Lactobacillus delbrueckii subsp. lactis and subsp. bulgaricus. Seventeen pathogenic bacterial isolates were subjected to the same analyses, with seven verified at the molecular level. Notably, Pseudomonas putida demonstrated multidrug resistance (MDR) against six antibiotics. and demonstrated strong Biofilm formation was assessed using two complementary methods Congo Red agar (CRA) and the microtiter plate assay. The antibacterial effects of Lactobacillus subsp. Lactis cell-free supernatants and bacterial pellets were tested against Pseudomonas putida using the well diffusion method. Results revealed that both Lactobacilli exerted significant inhibitory effects, with bacterial pellets showing greater activity than supernatants. These findings support the potential use of Lactobacillus delbrueckii subsp. Lactis and bulgaricus to combat MDR biofilm-forming pathogens, offering a promising alternative for infection control.

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Author Biographies

Haya Abdulkareem Albakry, Department of Biology, College of Education, University of Salahaddin, Erbil, IRAQ

Haya Abdulkareem Albakry is a Master’s student at the Department of Biology, College of Education, Salahaddin University Erbil, Iraq. She got the B.Sc. degree in Biology. Her research interests are in Microbiology, probiotics, and biofilm formation.
Sawsan Mohammed Sorchee, Department of Biology, College of Education, University of Salahaddin, Erbil, IRAQ

Assist. Prof. Sawsan Mohammed Sorchee has a Ph.D. degree in the field of Microbiology. Her research interests are in Microbiology, probiotics, and biofilm formation.

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Isolation and Identification of Lactobacillus delbrueckii from Cow and Sheep and its Effect on Pseudomonas putida Biofilm Formation