Cytotoxic Effects of Flaxseed (Linum usitatissimum L.) Aqueous Extract on Different Tumor Cell Lines: An in vitro Study: Cytotoxic Effects of Flaxseed (Linum usitatissimum L.) Aqueous Extract on Different Tumor Cell Lines

Reshna Kamal Albarzanji

doi:10.63841/iue24502

Authors

Reshna Kamal Albarzanji Department of Microbiology, Physiology, Genetics, College of Medicine, Hawler Medical University, Erbil 44001, IRAQ Author https://orcid.org/0000-0002-3772-5131

DOI:

https://doi.org/10.63841/iue24502

Keywords:

AMN-3 cell line; Aqueous Extract; Cytotoxic effect; Flaxseed; L20B; Rhabdomyosarcoma (RD) cell line

Abstract

Mortality rates from cancer are rising continuously worldwide, different methods are used to treat cancer such as chemotherapy, however, chemotherapy is cost ineffective and lacks selective toxicity, since it damages cancerous and healthy normal cells as well, therefore, seeking for natural alternatives is an insisted demand in clinical sitting. Medicinal plants and its derivatives are processed for the direct use as herbal or traditional medicine and attempted for clinical and experimental purposes. Flaxseed (Linum usitatissimum L.) is an oilseed utilized in industrial practice as well as natural health products. One of the major traditional interests in flaxseed was its important use in the prevention and treatment of many diseases, including cancer. The current study aimed to investigate the in vitro cytotoxic effect of flaxseed aqueous extract on tumor cell lines, and normal cell lines.

Two tumor cell lines (AMN3 and RD) and a normal cell line (L20B), at three time periods (24, 48, and 72 hrs of exposure) with different concentrations (0, 78.125, 156.25, 312.5 and 625µg/ml), were assessed.

Significant decrease in AMN3 cells viability was detected at the exposure period of 48 hrs in concentrations (156.25 (P= 0.002), 312.5 (P= 0.002) and 625 (P= 0.0004) μg/ml) except 78.125 µg/ml ((P= 0.360), of aqueous flaxseed extract were observed, significant decrease in AMN3 proliferation also observed at 72hrs of exposure in all concentrations of the extract (156.25 (P= 0.000), 312.5 (P= 0.002) and 625 (P= 0.005) μg/ml) except 78.125 µg/ml ((P= 0.328),which shows non -significant differences.

Concerning RD tumor cell line, all applied concentration (0,78.125, 156.25, 312.5, 625 μg/ml) revealed significant impact on the tumor cell lines at the three applied exposure period, without significant impact on the normal cell line (L20B).

Flaxseed has a concentration, and exposure period dependent impact on the AMN-3 and RD tumor cell lines, in vitro. Further studies are needed to detect this anti-proliferative effect and the pathway of flax seed extract in cancer treatment, both in vitro and in vivo.

Author Biography

Reshna Kamal Albarzanji, Department of Microbiology, Physiology, Genetics, College of Medicine, Hawler Medical University, Erbil 44001, IRAQ

Reshna Kamal Albarzanji Joined Hawler Medical University at 1992, She obtained M.Sc. in Cytology, Physiology & histology from Baghdad University/ Biology department-Baghdad (2002), She obtained Ph.D. in Cytogenetics from College of Medicine, Hawler Medical University-Erbil in 2012. She taught Cytology, Histology & genetics for Medical, pharmacy, and Medical Microbiology undergraduate and postgraduate students, and currently teaching Cytology and Molecular cell biology for undergraduate medical students and postgraduate student of Pharmacy and Biochemistry, and teaching Cytology and Histology, Embryology for Undergraduate Medical Microbiology students at International University of Erbil. She Published 4 papers in national and international journals. She Attended 10 national and international workshops and conferences. Her research interest: Cytology, Histology, Cytogenetics.

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Cytotoxic Effects of Flaxseed (Linum usitatissimum L.) Aqueous Extract on Different Tumor Cell Lines: An in vitro Study