Effects of Punica granatum Juice on Contractility of Isolated Aorta in Female Albino Rats
Effects of Punica granatum Juice on Contractility of Aorta
DOI:
https://doi.org/10.63841/iue11165Keywords:
Punica granatum juice, K+-channels blockers, Ca++-channels blocker, L-NAME, COX, Endothelium and hyperpolarizing factors.Abstract
The present study focused on the mechanisms of smooth muscle relaxation induced by Punica granatum juice (PI) in the rat thoracic aorta, The thoracic aorta was removed from female adult albino rat and placed in an organ bath containing Kreb's solution and the contractions were recorded isometrically. The results demonstrated that the P. granatum juice (1.5 to 5 mg/ml) significantly reduces the contractions induced by phenylephrine (PE) (0.01 mM), with IC50 ± SEM of 2.859 ± 0.101 mg/ ml, and percentage of relaxation for PE-induced contraction was 81.8
0.358. Also, the vasodilatory activity of PJ was not modified by endothelial denuded aorta with IC50 ± SEM 3.389 ± 0.084 mg/ml and the percentage of relaxation was 79.77 ±0.824%. Pomegranate juice induced relaxation in the PE-induced contraction in intact aorta was unaffected by nitric oxide synthase inhibitor (L-NAME) (3*10-4), gaunyl cyclase inhibitor (methylene blue) (1*10-5) and PGI2 inhibitor (Indometha-cin) (3*10-5) with IC50 ± SEM 3.424 ± 0.065, 3.286 ± 0.168 and 3.490 ± 0.054, respectively. The percentage of relaxation was 75.23 ± 0.661, 119,61 ± 4.128 and 83.87 ± 0.715%, respectively. In addition, Pre-in-cubation of aortic rings with TEA (1mM), GLIB (10-5), BaC12 (1mM) and 4-AP (1mM) with IC50 ‡ SEM 3.099 ± 0.049mg/ml, 2.759 ÷ 0.087 mg/ml, 2.889 + 0.054mg/ml and 2.436$0.049 mg/ml, and the percentages of relaxation were 101.38 0.151%, 88.55±0.58%, 90.25±0.168%, and 104.72÷0.939%, respectively. Finally, PJ significantly increased dose-response relaxation after incubation of thoracic aortic rings with Nifedipine (10-6 M) with IC50 ± SEM, 3.495 ± 0.1012 and the percentage of relaxation was 100.276 ± 3.378%.
References
Ajay, M., Gilani A. H. and Mustafa M. R., (2003). Effects of flavonoids on the vascular smooth muscle of the isolated rat thoracic aorta. Life Sciences. 74(5): 603-612.
AL-Habib, O.A.M. and Shekha M.S., (2010). Vasorelaxant Effect of Aqueous Extract of Crataegus azarolus aronia and Quercetin on Isolated Albino Rat's Thoracic Aorta. J. Duhok Univ. 13 (1): 7-13.
Anahita, A., Asmah R. and Fauziah O., (2015). Evaluation of total phenolic content, total antioxidant activity, and antioxidant vitamin composition of pomegranate seed and juice. General Medicine: Open Access, 2015.
Borochov-Neori, H., Judeinstein S., Tripler E., Harari M., Greenberg A., Shomer I. and Holland D., (2009). Seasonal and cultivar variations in antioxidant and sensory quality of pomegranate (Punica granatum L.) fruit. Journal of Food Composition and Analysis. 22(3): 189-195.
de Nigris, F., Balestrieri M. L., Williams-Ignarro S., D'Armiento F. P., Fiorito
C., Ignarro L. J. and Napoli C., (2007). The influence of pomegranate fruit extract in comparison to regular pomegranate juice and seed oil on nitric oxide and arterial function in obese Zucker rats. Nitric oxide. 17(1): 50-54.
Fulton, D., McGiff J. C. and Quilley )., 1996). Role of phospholipase C and phospholipase A2 in the nitric oxide-independent vasodilator effect of bradykinin in the rat perfused heart. Journal of Pharmacology and Experimental Therapeutics. 278(2): 518-526.
Herlekar, I., (2014). Fruit power: pomegranate helps fight Hepatitis C infec-tion. CURRENT SCIENCE. 107(7): 1105.Horak, E, Zieglmayer P., Ziegmayer R., Lemell P., Yao R., Staudinger H. and Danzig M., (2009). A placebo-controlled study of the nasal decongestant effect of phenylephrine and pseudoephedrine in the Vienna Challenge Cham-ber. Annals of Allergy, Asthma & Immunology. 102(2): 116-120.
Jorgensen, T. D., Dissing S. and Gromada, ]., (1996). Cyclic GMP potentiates phenylephrine but not cyclic ADP-ribose-evoked calcium release from rat lacrimal acinar cells. FEBS letters. 391(1-2): 117-120.
Kim, N. D., Mehta R., Yu W., Neeman I., Livney T., Amichay A., Poirier D., Nicholls P., Kirby A. and Jiang W., (2002). Chemopreventive and adjuvant therapeutic potential of pomegranate (Punica granatum) for human breast cancer. Breast cancer research and treatment. 71(3): 203-217.
Nakamura, Y., Matsumoto H. and Todoki K., (2002). Endothelium-dependent vasorelaxation induced by black currant concentrate in rat thoracic aorta. The Japanese journal of pharmacology. 89(1): 29-35.
Salleh, N. and Ahmad V. N., (2013). In-Vitro effect of Ficus deltoidea on the contraction of isolated rat's uteri is mediated via multiple receptors binding and is dependent on extracellular calcium. BMC complementary and alternative medicine. 13(1):1.
Shekha, M.S.S., (2010). Physiological Effects of Crataegus aronia Fractions on Isolated Smooth Muscle and Perfused "Langendorff" Heart in Albino Rats. PhD thesis. Duhok University. Kurdistan region-Iraq.
Sumner, M. D., Elliott-Eller M., Weidner G., Daubenmier ). J., Chew M. Н., Marlin R., Raisin C. J. and Ornish D., (2005). Effects of pomegranate juice consumption on myocardial perfusion in patients with coronary heart dis-ease. The American journal of cardiology. 96(6): 810-814.
Tzulker, R., Glazer I., Bar-Ilan I., Holland D., Aviram M. and Amir R., (2007). Antioxidant activity, polyphenol content, and related compounds in different fruit juices and homogenates prepared from 29 different pomegranate acces-sions. Journal of Agricultural and Food Chemistry. 55(23): 9559-9570.
Ugusman, A., Zakaria ., Chua K. H., Megat Mohd Nordin N. A. and Abdullah Mahdy Z., (2014). Role of rutin in nitric oxide synthesis in human umbilical vein endothelial cells. The Scientific World Journal. (2014).
Vanhoutte, P. M., (2009). Endothelial dysfunction the first step toward coro-nary arteriosclerosis. Circulation Journal. 73(4): 595-601.
Viuda-Martos, M., Fernández-López J. and Pérez-Álvarez J., (2010). Pomegranate and its many functional components as related to human health: a review. Comprehensive Reviews in Food Science and Food Safety. 9(6): 635-654.
Viuda-Martos, M., Ruiz-Navajas Y., Fernández-López )., Sendra E., Sayas- Bar-berá E. and Pérez-Álvarez J. A., (2011). Antioxidant properties of pomegranate (Punica granatum
L.) bagasses obtained co-product in the juice extraction. Food Research Inter-national. 44(5): 1217-1223.
Watson, R. R. and Preedy V. R., (2009). Bioactive foods in promoting health: fruits and vegetables: Academic Press.
Yilmaz, B. and Usta C., (2013). Ellagic Acid-Induced Endothelium-Dependent and Endothelium-Independent Vasorelaxation in Rat Thoracic Aortic Rings and the Underlying Mechanism. Phytotherapy Research. 27(2): 285-289.
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